Cláudio H. Zawadzki, Pedro Hollanda Carvalho, José L. O. Birindelli and Filipe M. Azevedo
Hypostomus nigrolineatus, new species, is described from the rio Jequitinhonha and rio Pardo basins. It is diagnosed by having dark spots over head, anterior portion of trunk and fins, and dark longitudinal stripes on flanks on posterior portion of body; incomplete scutelet coverage on abdomen in young specimens; 9 - 37 teeth on premaxilla, and 10 - 39 on dentary; dentary angled at approximately 90°; 25 -29 plates on median series; and moderate median keel of odontodes on most body plates.